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Evaluation of resistance and susceptibility level of single and double gene Bt cotton against Armyworm and American bollworm

Authors

  • Muhammad Amjad Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

  • Hafiza Masooma Naseer Cheema Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

  • Jam Nazeer Ahmad Department of Entomology, University of Agriculture, Faisalabad, Pakistan

  • Kashif Noor Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

  • Haram Aziz Alvi Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

  • Hanan Ahmad Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

  • Zobia Anwer Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

Keywords:

Bt cotton, Double gene ELISA, Insect bioassay, Lethal concentration

Abstract

Bt cotton was developed against lepidopteran insects and got a significant attraction by the Pakistani farmers to control target pests. The experiment was conducted to determine the resistance and susceptibility level of lepidopteran insects against double gene (Cry1Ac + Cry2A) and single genes Cry1Ac and Cry2A. Six Bt cotton genotypes G1 and G2 with Cry1Ac, G5 and G6 with Cry2A, and G3 and G4 with double gene (Cry1Ac + Cry2A) were studied. G7 was non-Bt which was used as a control. A qualitative test of Bt genes was done with immune-blot strips, and quantification was done by the ELISA technique. In the Armyworm bioassay, G1 and G2 showed 1.187 mg g-1 and 0.927 mg g-1 concentrations of Cry1Ac and 41.66% and 33.33% larval mortality, respectively. The Cry2A genotypes, G5 and G6, showed 7.60 mg g-1 and 7.79 mg g-1 concentrations of Cry2A, respectively, and larval mortality as 83.33% on both genotypes. The double gene genotypes, G3 and G4, showed Cry1Ac concentrations as 0.803 mg g-1 and 0.730 mg g-1, those of Cry2A 7.459 mg g-1 and 7.407 mg g-1, whereas the larval mortality of the two genotypes had been 91.67% and 100%, respectively. In the American bollworm bioassay, G1 and G2 showed concentration of Cry1Ac as 1.049 mg g-1 and 1.052 mg g-1 and larval mortality 33.33% and 41.66%, respectively. G5 and G6 showed Cry2A concentrations as 6.520 mg g-1 and 6.464 mg g-1 and the larval mortality levels as 66.67% and 58.33%, respectively. G3 and G4 showed concentrations of Cry1Ac and Cry2A as 1.460 mg g-1 and 0.968 mg g-1, and 6.025 mg g-1 and 6.651 mg g-1 and the larval mortality as 100% and 91.66%, respectively. Low weight gain was observed on those genotypes which contained double gene rather than any of the single genes. Strong positive correlations existed between insect’s mortality and double gene concentration. A lethal concentration (LC) of the double gene of Armyworm and American bollworm was 7.513 mg g-1 and 7.684 mg g-1, respectively, required to kill 99% of the insect’s population. Double gene Bt cotton genotypes had resistance against target pests, while single-gene Bt cotton genotypes lost their efficacy against the target pests.

Author Biographies

Hafiza Masooma Naseer Cheema, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

She is Associate Professor in University of Agriculture Fiasalabad, Pakistan.

Jam Nazeer Ahmad, Department of Entomology, University of Agriculture, Faisalabad, Pakistan

He is faculty member of entomology dapartment of university of agriculture faisalabad.

Kashif Noor, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

He is teaching assistant in plant breeding and gentics department of university of agriculture faisalabad, Pakistan

Haram Aziz Alvi, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

He done m.phil from university of agriculture faisalabad, Pakistan

Hanan Ahmad, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

He passed m.phil PBG from university of agriculture faisalabad, Pakistan

Zobia Anwer, Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan

She is working as lecturer in GSCW university bahawalpur.

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Published

2023-11-02

How to Cite

Amjad, M., Cheema, H. M. N., Ahmad, J. N., Noor, K., Alvi, H. A., Ahmad, H., & Anwer, Z. (2023). Evaluation of resistance and susceptibility level of single and double gene Bt cotton against Armyworm and American bollworm. International Journal of Applied and Experimental Biology, 1(1). Retrieved from http://submissions.eminentscientists.com/index.php/IJAEB/article/view/69

Issue

Section

Plant Biology