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DOI:

https://doi.org/10.56612/ijaeb.v1i1.23

Identification and reconstruction of phylogeny among three species of Cleome using nrDNA-ITS sequence

Authors

  • Salim Khan Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Fahad Al-Qurainy Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Mohammad Nadeem Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Mohamed Tarroum Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Saleh Alansi Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Abdel-Rhman Gaafar Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Aref Alshameri Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Hassan O. Shaikhaldein Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

  • Abdalrhaman M. Salih
  • Norah Arrak Alenezi Department of Botany and Microbiology, College of Science, King Saud University, Riyadh- 11451, Saudi Arabia

Keywords:

Molecular marker, Medicinal plants, ITS marker, PCR, sequencing

Abstract

Three species of genus Cleome, C. arabica, C. ramosissima and C. amblyocarpa were identified using nuclear ribosomal DNA internal transcribed spacer sequence (nrDNA-ITS). The phylogenetic tree was made using different methods viz., neighbor-joining (NJ), maximum parsimony (MP), and maximum likelihood for accuracy of the results, and  congruence finding in phylogeny. Cleome ramosissima and C. amblyocarpa were found to be clustered in one group according to the sequence similarity. However, C. arabica clustered in a separate clade. The nrDNA-ITS marker clearly differentiated and showed phylogenetic relationships among the three species. Thus, nrDNA-ITS marker could be effectively used for other species of Cleome for their identification and phylogenetic studies.

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Published

2022-11-15

How to Cite

Khan, S., Al-Qurainy, F., Nadeem, M., Tarroum, M., Alansi, S., Gaafar, A.-R., Alshameri, A., Shaikhaldein, H. O., Salih, A. M., & Alenezi, N. A. (2022). Identification and reconstruction of phylogeny among three species of Cleome using nrDNA-ITS sequence. International Journal of Applied and Experimental Biology, 2(1), 43–46. https://doi.org/10.56612/ijaeb.v1i1.23

Issue

Section

Plant Biology